Callus Induction of Beet Root for Speed up Economical Plant Production
Callus induction, one in plant tissue culture techniques, has become powerful method for speeding up plant production and solving the problems to apply in plant biotechnology. Callus induction of beet root was experimented in order to increase the plant production using in medical advantages. The suitable sterilization condition for beet root tissue and the appropriate amount of naphthaleneacetic acid (NAA) / N4- benzylaminopurine (BAP) ratio, portion of hormones to induce the forming of beet root callus, were investigated. Sterilization with 15% Clorox for 15 minutes was the best condition for beet root tissue. High percentage up to 55% of beet root survival was reached. This condition was applied before the beet root tissue was cultured on a Murashige and Skoog Basal Medium supplemented with a range of concentrations of NAA combined with BAP to optimize callus induction. The highest fresh weight (3,874.2 mg) of callus tissue occurred with 2 mg/L NAA combined with 0.5 mg/L BAP. Triphenyl tetrezolium chloride test showed that 100% survivability of callus tissue was obtained.